anti 8 ohdg antibody  (Bioss)

Journal: bioRxiv

Article Title: Neuroendocrine Stress Induces Differential Oxidative Stress and Antioxidant Profiles between Proactive and Reactive Stress Coping Styles

doi: 10.64898/2026.02.03.703382

Figure Lengend Snippet: Effects of acute stress on oxidative stress and antioxidant markers across stress coping styles. The boxplot shows (a) DNA damage, (b) glutathione ratio (c) ORAC, (d) total glutathione, and (e) SOD levels. The blue box indicates the baseline group of fish, while the yellow box represents the stressed group of fish. (*) represents p < 0.05 and (†) represents 0.05 < p < 0.10. Each dot represents a fish.

Article Snippet: DNA damage (8-OHdG) was measured with a DNA damage ELISA kit (StressMarq, Biosciences Inc., Victoria, BC, Canada) following manufacturer’s protocol.

Techniques:

Journal: bioRxiv

Article Title: Neuroendocrine Stress Induces Differential Oxidative Stress and Antioxidant Profiles between Proactive and Reactive Stress Coping Styles

doi: 10.64898/2026.02.03.703382

Figure Lengend Snippet: Composite measures (principal component scores) of oxidative stress and antioxidant biomarkers across stress coping styles. (a) Plots of principal components (b) SOD, total glutathione, and ORAC loaded onto principal component 1 (antioxidant axis), whereas (c) DNA damage and glutathione ratio loaded onto principal component 2 (oxidative stress axis). (*) represents p < 0.05 and (†) represents 0.05 < p < 0.10. Each dot represents a fish.

Article Snippet: DNA damage (8-OHdG) was measured with a DNA damage ELISA kit (StressMarq, Biosciences Inc., Victoria, BC, Canada) following manufacturer’s protocol.

Techniques:

Journal: Cell Communication and Signaling : CCS

Article Title: PPARγ agonism ameliorates acute kidney injury by inhibiting neutrophil extracellular trap formation-mediated renal tubular epithelial cell PANoptosis

doi: 10.1186/s12964-026-02686-6

Figure Lengend Snippet: CP-induced NETosis occurs through a ROS-dependent pathway. Inhibition of neutrophil ROS reduces CP-induced NETosis. A Detection of Cit-H3 expression and assessment of neutrophil death levels using live/dead cell staining and immunofluorescence microscopy. Scale bar, 100 μm. B Western blot analysis of P62 and LC3B expression in neutrophils. C Kidney tissue sections were stained for 8-OHdG, and representative images with corresponding quantitative histograms are shown. In addition, frozen kidney tissue sections were stained with a ROS detection solution to visualize ROS levels. Scale bar, 50 μm. D Quantification of kidney ROS levels using a fluorescence microplate reader. E Representative fluorescence images showing ROS production (detected by DHE staining) in neutrophils. Scale bar, 200 μm. F Detection of ROS levels in bone marrow-derived neutrophils using a fluorescence microplate reader after stimulation with CP or PMA. G Fluorescence microscopy imaging of Cit-H3 expression in neutrophils treated with NAC or chloroquine followed by CP exposure. Scale bar: 50 μm. NAC: N-acetyl-L-cysteine (antioxidant); CQ: chloroquine (autophagy inhibitor). Experiments were performed in triplicate, and the results are presented as mean ± SEM. Statistical significance was analyzed using independent samples t-tests ( C , D ) or one-way ANOVA, followed by Tukey’s post hoc test ( B , F ). Significance levels: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns: not significant

Article Snippet: The antibody against 8-OHdG was purchased from Bioss (bs1278R, China).

Techniques: Inhibition, Expressing, Staining, Immunofluorescence, Microscopy, Western Blot, Fluorescence, Derivative Assay, Imaging